Research Assistant In Crispr-Engineered Cellular Models Of Genetic Disorders

IIGM is located at the FPO IRCCS Candiolo Cancer Institute, Candiolo Turin - Italy. The main goal of FPO IRCCS Candiolo (https://www.irccs.com/en) and IIGM (Home - Italian Institute for Genomic Medicine) is to foster and develop excellence in research and education, with special reference to cancer biology and cancer biomarker discovery. The institute provides high-level scientific education for students and post‑doctoral fellows, and access to state-of-the-art research facilities and international networks.

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The Chemical Biology Lab at the Italian Institute for Genomic Medicine (IIGM) is seeking highly motivated candidates at all levels of seniority (predoc, postdoc) to work in high‑content imaging and analysis with a focus on cell‑to‑cell heterogeneity and phenotypic variability in membrane trafficking. The laboratory investigates key aspects of Rab GTPases and their involvement in hereditary genetic diseases. The project aims to generate a cellular model of steroid‑resistant nephrotic syndrome (SRNS) disease and perform genotype‑phenotype correlation analysis using molecular and computational tools developed in the lab. By integrating phenotypic mapping with biochemical and genetic information, we seek to elucidate the biological functions of rare genetic variants causing steroid‑resistant nephrotic syndrome. The institute provides state‑of‑the‑art genomics, single‑cell genomics sequencing platforms and high‑content imaging platforms.

The successful candidates will take on the following responsibilities:

• Generate isogenic HK‑2 cell lines harbouring genetic mutations causing SRNS disease
• Perform immunostaining of Rab GTPase and other proteins involved in intracellular trafficking
• Perform biochemical experiments on Rab GTPase regulators
• Conduct image acquisition using automated microscopy systems
• Perform image data analysis of high‑dimensional dataset
• Contribute to ongoing research group activities and collaborative projects

Qualifications and requirements:

• Bachelor’s degree in biotechnology, biology, medicine, chemical biology, pharmacy or a related field
• 1 year experience in at least one of the following subjects:
  • (i) Generation of knock‑in CRISPR/Cas9 edited human cells
  • (iii) High‑content imaging and data analysis
  • (iv) Mammalian tissue culture (cells and/or organoids)
• Good command of the English language
• Knowledge of Italian is an advantage, but not strictly necessary
• Curiosity and active learning
• Critical thinking and problem‑solving
• Time management
• Adaptability and flexibility
• Effective communication in cross‑cultural environments

Interested candidates should include:

• Research proposal (for postdoc applicants only)
• Certificates, transcripts including all grades of your previous qualifications, and a list of publications

Positions: 01

Offered contract: Coordinated and Continuous Collaboration contract (Co.Co.Co.) on a project basis duration to be defined. Competitive remuneration based on the candidate’s personal qualifications.

Research assistant in CRISPR‑engineered cellular models of genetic disorders • Candiolo, Torino, Italy

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